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The amount of fragments needs to be documented, the kind of biopsy, e.g., shave, needle or core biopsies, and whether or not the specimens characterize tissue as well as overseas substance. In processing the contents of a container, just one should document the quantity of fragments and check out not to work with conditions such as various or vario

The incubation time in liquid paraffin ought to be extensive plenty of to get satisfactory infiltration, but not any longer than required so that you can prevent prolonged publicity in the tissue to warmth. For a similar reason, it is recommended that the temperature on the liquid paraffin must be not more than 2–4 C previously mentioned the para

This can be especially correct for immunohistochemistry during which ten% NBF continues to be revealed for being a weak fixative (Fig. two). Some problems with using ten% NBF like a fixative have already been corrected because of the introduction of other experimental tactics like antigen retrieval (Shi et al. 2001) and using smaller amplicons in R

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